Plasmid_Backbone
Part:BBa_K4452010
Designed by: Juan Antonio Garcia, Fabio Pasin Group: iGEM22_Hopkins (2022-09-30)
pLX-B3Ω1
pLX series: pBBR1-based T-DNA binary vector for plant transformation (omega1)
Bacterial Resistance: Spectinomycin
GoldenBraid compatibility: enzyme in BsmBI, enzyme out BsaI.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal SpeI site found at 233
Illegal PstI site found at 622 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal NheI site found at 616
Illegal SpeI site found at 233
Illegal PstI site found at 622 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal SpeI site found at 233
Illegal PstI site found at 622 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal SpeI site found at 233
Illegal PstI site found at 622
Illegal NgoMIV site found at 954 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 253
Illegal BsaI.rc site found at 596
[edit]
Categories
Parameters
None |